视杆细胞外段膜：类视黄醇的良好受体？

Rod outer segment membranes: good acceptors for retinoids?

作者信息

Groenendijk G W, De Grip W J, Daemen F J

出版信息

Vision Res. 1984;24(11):1623-7. doi: 10.1016/0042-6989(84)90320-1.

Abstract

The exchange of all-trans retinoids (retinal, retinol, retinylpalmitate) between PC-vesicles, PC-vesicles and liver microsomes or PC-vesicles and rod outer segment membranes is investigated using 11,12(3)H labeled compounds. In the first two systems, retinal and retinol exchange rapidly, retinyl acetate slowly and retinyl palmitate not at all. Rod outer segment membranes however take up relatively small amounts of retinoids (retinylpalmitate less than retinol less than retinal) and rapidly lose 60-90% of their label in the presence of PC-vesicles. E.G. retinoids clearly favour the PC-vesicle membrane. Apparently, rod outer segment membranes have a much lower affinity for retinoids than other artificial or natural membranes investigated so far.

摘要

使用11,12(3)H标记的化合物研究了全反式视黄醇（视黄醛、视黄醇、棕榈酸视黄酯）在PC囊泡、PC囊泡与肝微粒体之间或PC囊泡与视杆外段膜之间的交换。在前两个体系中，视黄醛和视黄醇交换迅速，醋酸视黄酯交换缓慢，棕榈酸视黄酯根本不交换。然而，视杆外段膜摄取的类视黄醇相对较少（棕榈酸视黄酯＜视黄醇＜视黄醛），并且在存在PC囊泡的情况下会迅速失去60-90%的标记物。例如，类视黄醇明显倾向于PC囊泡膜。显然，视杆外段膜对类视黄醇的亲和力远低于迄今为止研究的其他人工或天然膜。