Seitelberger R, Raberger G
J Pharmacol Methods. 1984 Dec;12(4):233-46. doi: 10.1016/0160-5402(84)90009-3.
An experimental model of exercise-induced angina pectoris was developed in anesthetized dogs. Two i.v. bolus injections of isoproterenol (ISO, 0.25 microgram/kg; time interval: 10 min) were administered, followed by constriction of the circumflex coronary artery (LCX; critical stenosis). Further i.v. bolus injections of ISO were administered before and after i.v. infusion of 0.9% NaCl (control infusion). Stenosis of the LCX did not modify regional function at rest as assessed with ultrasonic crystals implanted subendocardially both in the distribution areas of the circumflex coronary artery and of the left anterior descending coronary artery (LAD). In the presence of critical stenosis, ISO induced a reproducible, transient decrease in systolic shortening and percent systolic shortening in the area supplied by the LCX and a marked increase in systolic shortening and percent systolic shortening in the area supplied by the LAD. During a 60-min observation period following control infusion, no further substantial changes in regional dimensions were observed. The ISO-induced hemodynamic changes were reproducible during this period. The experimental model of exercise-induced angina pectoris presented in this study can be assumed to provide a reliable basis for evaluating the efficacy of various therapeutical interventions.
在麻醉犬身上建立了运动诱发型心绞痛的实验模型。静脉推注两次异丙肾上腺素（ISO，0.25微克/千克；时间间隔：10分钟），随后对左旋冠状动脉（LCX）进行缩窄（临界狭窄）。在静脉输注0.9%氯化钠（对照输注）前后，进一步静脉推注ISO。如通过心内膜下植入的超声晶体评估，左旋冠状动脉狭窄并未改变左旋冠状动脉分布区域和左前降支冠状动脉（LAD）分布区域静息时的局部功能。在存在临界狭窄的情况下，ISO可导致左旋冠状动脉供血区域的收缩期缩短和收缩期缩短百分比出现可重复的短暂下降，以及左前降支冠状动脉供血区域的收缩期缩短和收缩期缩短百分比显著增加。在对照输注后的60分钟观察期内，未观察到局部尺寸有进一步的显著变化。在此期间，ISO诱导的血流动力学变化具有可重复性。本研究中提出的运动诱发型心绞痛实验模型可被认为能为评估各种治疗干预措施的疗效提供可靠依据。
