人类大脑：不同区域的醛脱氢酶活性及同工酶分布

Human brain: aldehyde dehydrogenase activity and isozyme distribution in different areas.

作者信息

Pietruszko R, Meier J, Major L F, Saini N, Manz H, Hawley R J

出版信息

Alcohol. 1984 Sep-Oct;1(5):363-7. doi: 10.1016/0741-8329(84)90004-1.

Abstract

Three human post-mortem brains were dissected into seventeen areas and assayed for aldehyde dehydrogenase (EC 1.2.1.3) activity employing two assay systems: one at 68 microM and another at 13.6 mM propionaldehyde. The levels of activity with 68 microM propionaldehyde were significantly higher in cerebellum and putamen. The same brain areas were also examined by isoelectric focusing. By this procedure two distinct bands of aldehyde dehydrogenase activity (the cytoplasmic E1 and mitochondrial E2) could be readily visualized in cerebellum and putamen while other brain areas contained mainly the mitochondrial E2 isozyme.

摘要

取三个人类尸检大脑，将其解剖为17个区域，使用两种检测系统测定醛脱氢酶（EC 1.2.1.3）活性：一种以68微摩尔/升丙醛为底物，另一种以13.6毫摩尔/升丙醛为底物。以68微摩尔/升丙醛为底物时，小脑和壳核中的酶活性水平显著更高。还通过等电聚焦对相同的脑区进行了检测。通过该方法，在小脑和壳核中可以很容易地观察到两条不同的醛脱氢酶活性带（细胞质E1和线粒体E2），而其他脑区主要含有线粒体E2同工酶。