Cameron I L, Hunter K E, Smith N K
Physiol Chem Phys Med NMR. 1984;16(6):521-5.
To determine if nuclear/cytoplasmic (N/C) gradients of ions exist in mammalian hepatocytes, as has been shown to exist in the amphibian oocyte, small pieces of mouse liver were cryofixed for energy dispersive X-ray microanalysis of Na, K and Cl in thin freeze-dried cryosections. Expressing the data on a dry weight basis showed small but significant N/C gradients for Na and K but not for Cl. When the data were expressed on a wet weight basis no significant N/C gradients of Na, K or Cl were revealed. Questions of data interpretation are discussed.
为了确定哺乳动物肝细胞中是否存在离子的核/质（N/C）梯度，正如在两栖类卵母细胞中已被证明存在的那样，将小块小鼠肝脏进行冷冻固定，以便对冻干薄冰冻切片中的钠、钾和氯进行能量色散X射线微分析。以干重为基础表示数据时，钠和钾显示出小但显著的N/C梯度，而氯则没有。以湿重为基础表示数据时，钠、钾或氯均未显示出显著的N/C梯度。文中讨论了数据解释的问题。
