Lambert H
J Reprod Fertil. 1984 Jan;70(1):281-4. doi: 10.1530/jrf.0.0700281.
In-vitro fertilization and sperm binding to the zona pellucida within and between Mus musculus (laboratory mouse) and Mus caroli (Asian wild field mouse) were examined. Maximum homologous sperm binding in M. musculus was 12.3 +/- 0.3 spermatozoa bound/egg and in M. caroli, 9.2 +/- 0.2. In heterologous crosses, sperm-zona binding was significantly reduced (M. musculus eggs with M. caroli spermatozoa, 1.9 +/- 0.14; M. caroli eggs with M. musculus spermatozoa, 5.5 +/- 0.2). Homologous sperm-zona interaction was inhibited by different sugars in the two mouse species (M. caroli by alpha-methyl mannose, M. musculus by sialic acid). The data suggest that gamete recognition is highly species specific and that hapten sugars play a significant role in sperm-zona binding.
对小家鼠（实验小鼠）和褐家鼠（亚洲野生田鼠）体内及两者之间的体外受精以及精子与透明带的结合进行了研究。小家鼠中最大同源精子结合数为12.3±0.3个精子/卵，褐家鼠中为9.2±0.2个。在异种杂交中，精子与透明带的结合显著减少（小家鼠卵与褐家鼠精子结合，1.9±0.14；褐家鼠卵与小家鼠精子结合，5.5±0.2）。两种小鼠中不同糖类抑制了同源精子与透明带的相互作用（褐家鼠中为α-甲基甘露糖，小家鼠中为唾液酸）。数据表明配子识别具有高度物种特异性，并且半抗原糖类在精子与透明带的结合中起重要作用。
