Kawai Y, Takemoto M, Oda Y, Kakehi K, Ohta Y, Yamaguchi S, Miyake M
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Biol Pharm Bull. 2000 Aug;23(8):936-40. doi: 10.1248/bpb.23.936.
The effect of sialic acid (N-acetyl neuraminic acid), sialic acid dimer, sialic acid polymers (colominic acid) and sulfated colominic acid on the activity of hyaluronidase, on the dispersion of cumulus cells by mouse sperm and on in vitro mouse fertilization (sperm penetration of zona pellucida) were evaluated. Bovine testicular hyaluronidase activity was significantly inhibited by colominic acid and sulfated colominic acid, but not by sialic acid and its dimer. The dispersion of cumulus cells from eggs by mouse sperm was also inhibited by colominic acid and sulfated colominic acid. In vitro fertilization of mouse gametes was inhibited by sulfated colominic acid. The IC50 value of sulfated colominic acid-induced inhibition of fertilization was 0.3 mg/ml (ca. 0.9 mM). The value changed from 0.9 mM for cumulus-surrounded egg to 1.5 mM for cumulus free-egg. On the other hand, colominic acid showed little or no inhibitory effect on mouse in vitro fertilization at 0.5 mg/ml (ca. 1.6 mM). This antifertility activity by sulfated colominic acid did not appear to be due to an effect on sperm motility or on the oocytes. These results suggest that (1) the cumulus cells surrounding the eggs were dispersed by sperm hyaluronidase, (2) hyaluronidase was inhibited by colominic acid and by sulfated colominic acid, (3) sulfated colominic acid inhibits sperm penetration of zona pellucida by the inhibition of hyaluronidase and/or some enzymes required for mouse gametes fertilization.
评估了唾液酸（N-乙酰神经氨酸）、唾液酸二聚体、唾液酸聚合物（结肠粘多糖酸）和硫酸化结肠粘多糖酸对透明质酸酶活性、小鼠精子对卵丘细胞的分散作用以及体外小鼠受精（精子穿透透明带）的影响。结肠粘多糖酸和硫酸化结肠粘多糖酸可显著抑制牛睾丸透明质酸酶的活性，但唾液酸及其二聚体则无此作用。结肠粘多糖酸和硫酸化结肠粘多糖酸也可抑制小鼠精子对卵丘细胞的分散作用。硫酸化结肠粘多糖酸可抑制小鼠配子的体外受精。硫酸化结肠粘多糖酸诱导受精抑制的IC50值为0.3mg/ml（约0.9mM）。该值从对有卵丘包围的卵的0.9mM变为对无卵丘的卵的1.5mM。另一方面，结肠粘多糖酸在0.5mg/ml（约1.6mM）时对小鼠体外受精几乎没有抑制作用。硫酸化结肠粘多糖酸的这种抗生育活性似乎不是由于对精子活力或卵母细胞的影响。这些结果表明：（1）包围卵的卵丘细胞被精子透明质酸酶分散；（2）透明质酸酶被结肠粘多糖酸和硫酸化结肠粘多糖酸抑制；（3）硫酸化结肠粘多糖酸通过抑制透明质酸酶和/或小鼠配子受精所需的某些酶来抑制精子穿透透明带。
