Takada A, Takada Y, Sugawara Y
Thromb Res. 1984 Mar 15;33(6):561-9. doi: 10.1016/0049-3848(84)90111-7.
Glu- and Lys-plasminogen (plg) were mixed with fibrinogen or fibrinogen plus thrombin in the presence of various units of urokinase (UK) and S-2251 (H-D-Val-Leu-Lys-pNA). Time course of the hydrolysis of S-2251 and the increment of OD405/min were monitored by using a spectrophotometer. Glu-plg was activated better by UK in the presence of fibrinogen and fibrin than in their absence. The effects of fibrin were larger than those of fibrinogen in the activation of Glu-plg. When Lys-plg was used for the similar experiments, the activation rate of Lys-plg was slightly increased in the presence of fibrinogen and fibrin (fibrin greater than fibrinogen), but their effects were smaller than in the case of the activation of Glu-plg.
在不同单位的尿激酶（UK）和S-2251（H-D-缬氨酸-亮氨酸-赖氨酸-对硝基苯胺）存在的情况下，将谷氨酸纤溶酶原（Glu-plg）和赖氨酸纤溶酶原（Lys-plg）与纤维蛋白原或纤维蛋白原加凝血酶混合。使用分光光度计监测S-2251水解的时间进程和每分钟OD405的增量。在有纤维蛋白原和纤维蛋白存在时，Glu-plg被UK激活的效果比不存在时更好。在激活Glu-plg方面，纤维蛋白的作用大于纤维蛋白原。当使用Lys-plg进行类似实验时，在有纤维蛋白原和纤维蛋白存在时（纤维蛋白的作用大于纤维蛋白原），Lys-plg的激活率略有增加，但它们的作用小于激活Glu-plg的情况。
