Takada A, Shizume K, Cho M, Takada Y
Thromb Res. 1987 Feb 15;45(4):371-81. doi: 10.1016/0049-3848(87)90226-x.
The activation of a native form of plasminogen (Glu-plg) by tissue plasminogen activator(t-PA) was enhanced when the plasma was clotted by the addition of thrombin or thrombin plus Ca++. Cross-linking of fibrin in the clotted plasma did not inhibit the fibrin-associated enhancement of the activation of plasminogen by t-PA. When fibrinolysis induced by t-PA in the clotted plasma was measured using enzyme immunoassay, lysis of non cross-linked fibrin in the clotted plasma was faster than lysis of cross-linked fibrin, however such decrease in the extent of fibrinolysis was observed in cross-linked fibrin even in the absence of alpha 2antiplasmin (alpha 2AP) in a purified system. When Glu- or Lys-plg (modified plg) was activated by t-PA, the presence of fibrin enhanced significantly the extent of activation of both Glu- and Lys-plg, but the activation of Glu-plg by urokinase (UK) was enhanced in the presence of fibrin. The activation of Lys-plg by UK was rather inhibited in the presence of fibrin.
当通过添加凝血酶或凝血酶加钙离子使血浆凝固时，组织型纤溶酶原激活剂（t-PA）对天然形式纤溶酶原（Glu-plg）的激活作用增强。凝血血浆中纤维蛋白的交联并不抑制t-PA对纤溶酶原激活的纤维蛋白相关增强作用。当使用酶免疫测定法检测t-PA在凝血血浆中诱导的纤维蛋白溶解时，凝血血浆中非交联纤维蛋白的溶解速度比交联纤维蛋白快，然而，即使在纯化系统中不存在α2抗纤溶酶（α2AP）的情况下，交联纤维蛋白中也观察到了这种纤维蛋白溶解程度的降低。当t-PA激活Glu-或Lys-plg（修饰的plg）时，纤维蛋白的存在显著增强了Glu-和Lys-plg的激活程度，但在纤维蛋白存在的情况下，尿激酶（UK）对Glu-plg的激活作用增强。在纤维蛋白存在的情况下，UK对Lys-plg的激活作用受到抑制。
