Dascal N, Yekuel R, Oron Y
J Exp Zool. 1984 Apr;230(1):131-5. doi: 10.1002/jez.1402300117.
Progesterone-induced maturation of follicle-enclosed and denuded Xenopus laevis oocytes was significantly shortened by a concomitant exposure to acetylcholine. The promotion of maturation by acetylcholine was blocked by the specific muscarinic antagonist atropine. The action of acetylcholine was dose dependent, and the neurotransmitter was effective at very low concentrations. Progesterone progressively reduced the electrophysiological responses of X. laevis oocytes to acetylcholine, which completely disappeared close to the time of germinal vesicle breakdown. Progesterone alone did not elicit any electrophysiological responses. The in vitro effect of acetylcholine on oocyte maturation might reflect a physiological influence of the cholinergic system on an in vivo maturation process.
同时暴露于乙酰胆碱会显著缩短孕酮诱导的非洲爪蟾卵泡包被卵母细胞和裸卵母细胞的成熟时间。乙酰胆碱对成熟的促进作用被特异性毒蕈碱拮抗剂阿托品阻断。乙酰胆碱的作用呈剂量依赖性，且该神经递质在极低浓度下就有效。孕酮逐渐降低非洲爪蟾卵母细胞对乙酰胆碱的电生理反应，在生发泡破裂时该反应完全消失。单独使用孕酮不会引发任何电生理反应。乙酰胆碱对卵母细胞成熟的体外作用可能反映了胆碱能系统对体内成熟过程的生理影响。
