肌动蛋白与肾上腺髓质嗜铬颗粒及线粒体组分的结合。
Binding of actin to chromaffin granules and mitochondrial fractions of adrenal medulla.
作者信息
Kao L S, Westhead E W
出版信息
FEBS Lett. 1984 Jul 23;173(1):119-23. doi: 10.1016/0014-5793(84)81029-7.
PMID:6540205
Abstract
Binding of actin to chromaffin granules was confirmed and shown to be salt dependent and eliminated by prior trypsin treatment of the granules. However, purified granules bind less actin than do crude granules. A mitochondria-enriched fraction was found to bind substantially more actin per mg protein than did the secretory vesicle fraction. Binding of actin by the secretory vesicles therefore is not a good indication that actin plays an active role in exocytosis.
摘要
已证实肌动蛋白与嗜铬颗粒结合，且这种结合依赖于盐，并且通过事先用胰蛋白酶处理颗粒可消除这种结合。然而，纯化的颗粒比粗颗粒结合的肌动蛋白更少。发现富含线粒体的部分每毫克蛋白质结合的肌动蛋白比分泌囊泡部分多得多。因此，分泌囊泡对肌动蛋白的结合并不能很好地表明肌动蛋白在胞吐作用中发挥积极作用。
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