Cohen H A, Jeng T W, Grant R A, Chiu W
Ultramicroscopy. 1984;13(1-2):19-25. doi: 10.1016/0304-3991(84)90053-6.
Technical factors which influence the choice of specimen preparation method for electron crystallographic study of thin crystals of soluble proteins are discussed. Ice embedding appears to be the most desirable choice of preparation method. However, in terms of the yield of major structural information, we conclude that negative stain remains a useful method for low resolution and glucose embedding for high resolution.
