Gala R R, Clarke W P, Haisenleder D J, Pan J T, Pieper D R
Endocrinology. 1984 Oct;115(4):1256-61. doi: 10.1210/endo-115-4-1256.
The influence of blinding (BLD), olfactory bulbectomy (ANOS), and pinealectomy (PX) on 24-h plasma PRL levels was examined in normal and neonatally androgenized (NA) female rats. NA pups were treated at 3 days of age with 1.25 mg testosterone propionate. Surgery was performed on both NA and normal animals at 22-25 days of age. At 10 weeks of age, animals were vaginally smeared, and at 15 weeks, they were ovariectomized, injected with 0.5 mg polyestradiol phosphate, and fitted with atrial catheters for blood sampling. At 16 weeks of age, blood samples were taken over a 24-h period. In normal animals, BLD plus ANOS resulted in half of the animals exhibiting a diestrous vaginal smear, while the other half exhibited at least one vaginal estrus during the 7-day period when vaginal smears were obtained. Ovarian, oviductal, and uterine weights for blinded (BLD) plus olfactory bulbectomized (ANOS) animals exhibiting diestrous smears were significantly less than those of BLD plus ANOS animals exhibiting periodic vaginal estrus. Plasma PRL levels were lower in both animal groups compared with those in sham controls, but BLD plus ANOS animals with small ovaries had significantly lower plasma PRL and BLD plus ANOS animals with large ovaries. PX of BLD plus ANOS animals resulted in endocrine organ weights comparable to those in controls and elevated the plasma PRL levels to those observed in BLD plus ANOS animals with large ovaries. The PRL levels in BLD, ANOS, and pinealectomized animals, however, were still below those in sham control animals. Sensory deprived animals had peak PRL values at various times of day, and regrouping of the data from the peak PRL time for each animal suggested the presence of a free-running plasma PRL rhythm. In NA animals, a decrease was also observed in endocrine organ weights and plasma PRL for BLD plus ANOS animals similar to that observed for normal BLD plus ANOS animals. However, all animals in the NA BLD plus ANOS group responded with a constant diestrous smear, small ovaries, and low plasma PRL values. PX prevented the decrease in endocrine organ weights and plasma PRL observed in BLD plus ANOS animals. As in normal animals, NA animals also exhibited a free-running PRL rhythm when sensory deprived.(ABSTRACT TRUNCATED AT 400 WORDS)
在正常和新生期雄激素化（NA）的雌性大鼠中，研究了致盲（BLD）、嗅球切除（ANOS）和松果体切除（PX）对24小时血浆催乳素（PRL）水平的影响。NA幼崽在3日龄时用1.25毫克丙酸睾酮进行处理。在22 - 25日龄时，对NA和正常动物都进行了手术。在10周龄时，对动物进行阴道涂片检查，在15周龄时，对它们进行卵巢切除，注射0.5毫克聚磷酸雌二醇，并安装心房导管用于采血。在16周龄时，在24小时内采集血样。在正常动物中，BLD加ANOS导致一半的动物呈现动情间期阴道涂片，而另一半在获取阴道涂片的7天期间至少出现一次阴道发情。呈现动情间期涂片的致盲（BLD）加嗅球切除（ANOS）动物的卵巢、输卵管和子宫重量显著低于呈现周期性阴道发情的BLD加ANOS动物。与假手术对照组相比这两组动物的血浆PRL水平都较低，但卵巢小的BLD加ANOS动物的血浆PRL显著更低，而卵巢大的BLD加ANOS动物则不然。对BLD加ANOS动物进行PX导致内分泌器官重量与对照组相当，并使血浆PRL水平升高到在卵巢大的BLD加ANOS动物中观察到的水平。然而，BLD、ANOS和松果体切除动物的PRL水平仍低于假手术对照动物。感觉剥夺的动物在一天中的不同时间有PRL峰值，对每只动物PRL峰值时间的数据重新分组表明存在自由运行的血浆PRL节律。在NA动物中，观察到BLD加ANOS动物的内分泌器官重量和血浆PRL也有下降，类似于在正常BLD加ANOS动物中观察到的情况。然而，NA BLD加ANOS组的所有动物都表现为持续的动情间期涂片、小卵巢和低血浆PRL值。PX阻止了在BLD加ANOS动物中观察到的内分泌器官重量和血浆PRL的下降。与正常动物一样，NA动物在感觉剥夺时也表现出自由运行的PRL节律。（摘要截断于400字）
