Aging and daily rhythms of analgesia in mice: effects of natural illumination and twilight.

Day-night rhythms in aversive thresholds and morphine-induced analgesia were assessed in young (1-3 months) and old (22-30 months) male mice exposed to natural summer (43 degrees N lat.) lighting using a hot-plate technique. In both age groups peak aversive thresholds and morphine-induced analgesia were present at night, with significantly reduced response times in the day. Response times increased during the course of the day with maximum increases in aversive thresholds and analgesia occurring during the decreasing light levels of dusk. Maximum decreases in the nocturnal response times occurred during the increasing light levels of dawn. The old mice displayed significantly lower nocturnal aversive thresholds and morphine-induced analgesia, as well as less pronounced patterns of change in response times during the dawn and dusk twilight lighting transitions. No significant effects of age were evident during the day-time.