Kjellstrand P T
J Histochem Cytochem. 1977 Feb;25(2):129-34. doi: 10.1177/25.2.65418.
Exposure and removal of aldehyde groups during Feulgen acid hydrolysis were studied at a wide range of temperature and acid concentrations. Temperatures between 9 and 75degreesC were found to influence only the rate of the hydrolysis reaction over the entire range from high (6 M) to low (0.05 M) HCl concentrations. The temperature dependence was high, and around +5degreesC was sufficient to double the reaction rate. The influence of acid concentrations between 0.02 and 6 M was studied, and the extraction rates that determine the peak values of the Feulgen hydrolysis curve were found to depend in the same way on the (H+) concentration. A diagram is given that makes it possible to determine the time to reach the point during hydrolysis where the maximum amount of aldehyde groups are developed for a wide range of temperatures and acid concentrations. Temperatures slightly above room temperature in combination with high acid concentration is recommended for Feulgen hydrolysis.
在广泛的温度和酸浓度范围内，研究了福尔根酸水解过程中醛基的暴露和去除情况。发现在9至75摄氏度之间的温度仅在从高（6 M）到低（0.05 M）的HCl浓度的整个范围内影响水解反应的速率。温度依赖性很高，大约+5摄氏度足以使反应速率加倍。研究了0.02至6 M之间的酸浓度的影响，发现决定福尔根水解曲线峰值的提取率以相同方式取决于（H +）浓度。给出了一个图表，通过该图表可以确定在广泛的温度和酸浓度范围内水解过程中达到醛基产生量最大点的时间。建议福尔根水解采用略高于室温的温度与高酸浓度相结合的方式。
