盐酸水解对DNA中胸腺嘧啶核苷保留率的影响。

The effect of HCl hydrolysis on the retention of thymidine in DNA.

作者信息

SAVAGE R E, PLAUT W

出版信息

J Biophys Biochem Cytol. 1958 Nov 25;4(6):701-6. doi: 10.1083/jcb.4.6.701.

DOI:10.1083/jcb.4.6.701

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2224516/

Abstract

Allium roots grown in C(14)-thymidine and H(3)-thymidine media were treated with N hydrochloric acid at 60 degrees C. as in standard Feulgen hydrolysis. The retention of the radioactive thymidine in DNA as a function of hydrolysis time was studied autoradiographically. No significant loss of label was detected until hydrolysis was extended beyond the optimal time for Feulgen staining. The data are consistent with the assumption that there is no significant loss of DNA during normal Feulgen hydrolysis in the material used.

摘要

将在含有C(14)-胸苷和H(3)-胸苷的培养基中生长的葱属植物根，按照标准福尔根水解法，在60摄氏度下用N盐酸处理。通过放射自显影法研究了作为水解时间函数的放射性胸苷在DNA中的保留情况。在水解时间延长至超过福尔根染色的最佳时间之前，未检测到标记的显著损失。这些数据与以下假设一致：在所使用的材料中，正常福尔根水解过程中DNA没有显著损失。

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本文引用的文献

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J Biophys Biochem Cytol. 1957 Jan 25;3(1):71-88. doi: 10.1083/jcb.3.1.71.

2

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