Tracy B M, Doremus R H
J Biomed Mater Res. 1984 Sep;18(7):719-26. doi: 10.1002/jbm.820180702.
The bone-hydroxylapatite interface has been examined directly in the transmission electron microscope (TEM). The bone-hydroxylapatite interface was characterized by using several electron microscopy techniques, including bright and dark field imaging, electron diffraction, high-resolution imaging, and energy dispersive analysis in the scanning-transmission electron microscope (STEM EDS). Mechanical grinding followed by argon-ion milling produced interface regions of unstained and undecalcified rat bone and hydroxylapatite that were transparent to electrons. Thus the exact location of the interface could be established and the bone at the interface studied. Conventional and high-resolution imaging in the TEM demonstrated direct chemical bonding between bone and hydroxylapatite. The bone at the ceramic surface was the same as normal bone away from the interface.
已在透射电子显微镜（TEM）中直接检查了骨-羟基磷灰石界面。通过使用几种电子显微镜技术对骨-羟基磷灰石界面进行了表征，包括明场和暗场成像、电子衍射、高分辨率成像以及扫描透射电子显微镜（STEM EDS）中的能量色散分析。机械研磨后进行氩离子铣削，产生了未染色和未脱钙的大鼠骨与羟基磷灰石的界面区域，这些区域对电子是透明的。因此，可以确定界面的确切位置，并对界面处的骨进行研究。TEM中的常规成像和高分辨率成像证明了骨与羟基磷灰石之间存在直接化学键合。陶瓷表面的骨与远离界面的正常骨相同。
