Dylewski D P, Haralick R M, Keenan T W
J Ultrastruct Res. 1984 Apr;87(1):75-85. doi: 10.1016/s0022-5320(84)90117-5.
The three-dimensional ultrastructure of the Golgi apparatus in milk secreting epithelial cells of bovine mammary gland was explored. From computer-aided reconstructions of serial thin sections, it was determined that the Golgi apparatus was composed of a single set of stacked cisternae. The three-dimensional shape of the dictyosome varied from cell to cell, but the overall shape was that of a hollow cone, cylinder, or bowl. The cis and trans surfaces of the dictyosome were arranged in three-dimensional space such that the cis face was located on the outer surface of the hollow structure and the trans face on the inner surface. The cytoplasmic channel (secretory channel) that traversed the longitudinal axis of the hollow dictyosome contained secretory vesicles. Densely stacked cisternae of rough endoplasmic reticulum surrounded the dictyosome, and microvesicles appeared to fuse with, or bud from, cisternae of both organelles. These findings suggest that Golgi apparatus of the lactating epithelial cell is highly organized and that the Golgi apparatus and secretory channel are essentially an independent compartment within the cell.
对牛乳腺泌乳上皮细胞中高尔基体的三维超微结构进行了探索。通过对连续薄切片的计算机辅助重建，确定高尔基体由一组堆叠的扁平囊组成。高尔基体的三维形状因细胞而异，但总体形状为空心圆锥体、圆柱体或碗状。高尔基体的顺面和反面在三维空间中排列，使得顺面位于空心结构的外表面，反面位于内表面。穿过空心高尔基体纵轴的细胞质通道（分泌通道）含有分泌小泡。密集堆叠的粗面内质网扁平囊围绕着高尔基体，微泡似乎与这两种细胞器的扁平囊融合或从其出芽。这些发现表明，泌乳上皮细胞的高尔基体高度有序，并且高尔基体和分泌通道在细胞内本质上是一个独立的区室。
