Structural study of tetragonal-ordered aggregates of phage phi 29 necks.

A new class of two-dimensional tetragonal aggregates of phage phi 29 necks has been studied by electron microscopy and a combination of Fourier filtering procedures and detailed rotational analysis. The results confirm the main features of the head-to-tail connecting region previously observed in hexagonal aggregates. There are several differences in the resulting pictures that can be attributed to the different way in which the aggregates are organized and stained.