Kachi T, Quay W B
J Pineal Res. 1984;1(2):163-74. doi: 10.1111/j.1600-079x.1984.tb00207.x.
Glycogen level in and size of pinealocytes of the feral, white-footed mouse Peromyscus leucopus, were studied by a semiquantitative histochemical method to determine whether seasonal changes exist in them under natural conditions, what temporal pattern they exhibit, and whether 24-hour changes in these parameters exist in different seasons, as shown in the laboratory dd-mice. Marked seasonal changes were seen in both glycogen levels and nuclear densities (ANOVA p less than 0.005). The size of pinealocytes at 09:00 to 10:00 showed one peak (and nadir) seasonal change, with the smallest size in winter (December and February) and a larger size in warmer seasons, with the maximum value in July. Glycogen level in pinealocytes at 09:00 to 10:00 showed bimodal seasonal changes, with lower levels in fall and spring and higher levels in winter and summer. In fall, a circadian trend in glycogen level in pinealocytes was seen, with a higher level at the end of the light period. In winter, the glycogen levels were very high at 09:00, 13:00, 17:00, and 21:00 examined and showed dampening of time-of-day differences. On the other hand, the size of pinealocytes followed a time-of-day change (P less than 0.005), being largest at 13:00 and smallest at 21:00. Thus, marked changes in quantitative structure and chemical activities, suggesting changes in functional activity, in pinealocytes were noted especially in severe, cold winter.
采用半定量组织化学方法研究了野生白足鼠（Peromyscus leucopus）松果体细胞中的糖原水平和大小，以确定在自然条件下它们是否存在季节性变化、呈现何种时间模式，以及这些参数在不同季节是否像实验室dd小鼠那样存在24小时变化。结果发现糖原水平和核密度均有明显的季节性变化（方差分析p<0.005）。09:00至10:00时松果体细胞大小呈现单峰（和最低点）季节性变化，冬季（12月和2月）最小，温暖季节较大，7月达到最大值。09:00至10:00时松果体细胞中的糖原水平呈现双峰季节性变化，秋季和春季较低，冬季和夏季较高。秋季，松果体细胞中的糖原水平呈现昼夜节律趋势，光照期结束时水平较高。冬季，在检测的09:00、13:00、17:00和21:00时糖原水平非常高，且昼夜差异减弱。另一方面，松果体细胞大小呈现昼夜变化（P<0.005），13:00时最大，21:00时最小。因此，尤其在严寒的冬季，观察到松果体细胞的定量结构和化学活性有明显变化，这表明其功能活性发生了改变。
