包含编码区和一个小内含子的人腺苷脱氨酶cDNA序列。
Sequence of human adenosine deaminase cDNA including the coding region and a small intron.
作者信息
Wiginton D A, Adrian G S, Hutton J J
出版信息
Nucleic Acids Res. 1984 Mar 12;12(5):2439-46. doi: 10.1093/nar/12.5.2439.
Abstract
The nucleotide sequence for an unusual, cloned human adenosine deaminase cDNA has been determined. Contained within a sequence of 1535 nucleotides is a coding sequence of 1089 nucleotides that encodes a protein of 40,762 daltons. The coding sequence is interrupted by a non-coding region containing 76 nucleotides. Both the 3' and 5' ends of this region have consensus sequences generally associated with splice sites. The 3' untranslated sequence contained 308 nucleotides, including a polyadenylation signal sequence 20 nucleotides from the end. The cloned cDNA appears to correspond to a nuclear mRNA precursor which contains a small intron.
摘要
已确定一个异常的克隆人腺苷脱氨酶cDNA的核苷酸序列。在1535个核苷酸的序列中包含一个1089个核苷酸的编码序列，该序列编码一个40762道尔顿的蛋白质。编码序列被一个包含76个核苷酸的非编码区域中断。该区域的3'端和5'端都有通常与剪接位点相关的共有序列。3'非翻译序列包含308个核苷酸，包括距末端20个核苷酸处的一个聚腺苷酸化信号序列。克隆的cDNA似乎对应于一个含有小内含子的核mRNA前体。
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