琼脂糖中趋化剂浓度分布和扩散系数的测量。

Measurement of chemoattractant concentration profiles and diffusion coefficient in agarose.

作者信息

Stickle D F, Lauffenburger D A, Zigmond S H

出版信息

J Immunol Methods. 1984 May 11;70(1):65-74. doi: 10.1016/0022-1759(84)90390-9.

Abstract

Migration of leukocytes is influenced by both the concentration and gradient of a chemoattractant. In the under-agarose assay, attractant diffuses through the medium away from the attractant well. For complete analysis of assay data, the diffusion coefficient of the attractant in the assay system must be known, and the attractant concentration profiles must be mathematically predictable. This paper describes a method whereby the diffusion coefficient of a chemoattractant through agarose may be determined, by measurement of attractant concentration profiles. The diffusion coefficient of FNLLP was found to be D = 1.0 X 10(-5) cm2/s at 37 degrees C. In addition it was found that the concentration profiles can be satisfactorily predicted by solution of the diffusion equation with appropriate boundary conditions.

摘要

白细胞的迁移受趋化因子的浓度和梯度影响。在琼脂糖下测定中，趋化因子从趋化因子孔向培养基中扩散。为了对测定数据进行完整分析，必须知道趋化因子在测定系统中的扩散系数，并且趋化因子浓度分布必须在数学上是可预测的。本文描述了一种通过测量趋化因子浓度分布来确定趋化因子在琼脂糖中扩散系数的方法。发现FNLLP在37℃时的扩散系数为D = 1.0×10^(-5) cm²/s。此外，还发现通过在适当边界条件下求解扩散方程，可以令人满意地预测浓度分布。