Allen J M, Yeats J C, Adrian T E, Bloom S R
Regul Pept. 1984 Jan;8(1):61-70. doi: 10.1016/0167-0115(84)90029-6.
The development of a radioimmunoassay to the newly isolated peptide, neuropeptide Y is described. Four separate antisera have been developed using different immunisation schedules. Two of these antisera (YNI and YNIO) are directed to the C-terminal region of the peptide and cross-react with the related peptide PYY, whereas YN7 is specific being directed to the N-terminal region of NPY, YN6 is similarly specific for NPY, but is unable to bind the available fragments. These four antisera provide similar results for determination of NPY immunoreactivity within porcine brain extracts, however YN6 consistently undervalues all extracts from the other species examined (human, rat, guinea pig, cat and mouse). Chromatographic analysis by means of reverse phase high pressure liquid chromatography (HPLC) shows that NPY immunoreactivity of human extracts elutes in an earlier position than the porcine standard. It seems likely therefore that human and porcine NPY differ in their amino acid sequences.
本文描述了针对新分离的肽神经肽Y开发放射免疫测定法的过程。使用不同的免疫方案制备了四种不同的抗血清。其中两种抗血清（YNI和YNIO）针对该肽的C末端区域，与相关肽PYY发生交叉反应，而YN7特异性针对NPY的N末端区域，YN6同样对NPY具有特异性，但无法结合可用片段。这四种抗血清在测定猪脑提取物中的NPY免疫反应性时提供了相似的结果，然而，YN6始终低估了所有来自其他检测物种（人、大鼠、豚鼠、猫和小鼠）的提取物。通过反相高压液相色谱（HPLC）进行的色谱分析表明，人提取物的NPY免疫反应性比猪标准品更早洗脱。因此，人和猪的NPY氨基酸序列似乎存在差异。
