Lundberg J M, Terenius L, Hökfelt T, Tatemoto K
J Neurosci. 1984 Sep;4(9):2376-86. doi: 10.1523/JNEUROSCI.04-09-02376.1984.
Antisera raised against porcine neuropeptide Y (NPY) and peptide YY (PYY) were characterized with regard to immunohistochemical staining, cross-reactivity to several pancreatic polypeptide (PP)-related peptides, and radioimmunoassayable tissue levels in the rat and pig. The NPY antiserum (102B) reacted with nerves in many areas of both the central and peripheral nervous systems, but it did not stain endocrine cells of the pancreas or intestine. No evidence for any cross-reactivity of the NPY antiserum with related peptides of the PP family, such as avian PP, bovine PP, PYY, gamma-MSH, FMRF-amide, or avian PP (31-36), was obtained. The NPY antiserum was N-terminally directed, and regional levels of NPY as seen by radioimmunoassay paralleled well the occurrence of NPY-immunoreactive structures seen in the immunohistochemical study. High pressure liquid chromatography analysis revealed that the NPY-immunoreactive material from cerebral cortex and vas deferens had elution profiles similar to those of standard porcine NPY. The PYY antiserum mainly stained endocrine cells in the pancreas and intestine as well as a small neuron system in the brainstem of the rat. Although this antiserum had a slight cross-reactivity to NPY in radioimmunoassay, the neuronal PYY staining was separate from that of NPY. High levels of PYY were found in the intestine, and levels above the threshold were also seen in the dorsal vagal complex of the rat. The other antisera investigated (raised against avian PP, bovine PP, gamma-MSH, and FMRF-amide) caused neuronal staining that was abolished by preabsorption with NPY. This was also seen even if no detectable cross-reactivity with NPY was found in radioimmunoassay. These latter antisera also stained endocrine cells in the pancreas and intestine with complex cross-reactivity relationships, suggesting the presence of intestinal PP-like peptides in addition to PYY and NPY.
针对猪神经肽Y（NPY）和肽YY（PYY）制备的抗血清，在大鼠和猪身上进行了免疫组织化学染色、对几种胰多肽（PP）相关肽的交叉反应性以及放射免疫测定的组织水平等方面的特性研究。NPY抗血清（102B）与中枢和外周神经系统许多区域的神经发生反应，但不使胰腺或肠道的内分泌细胞染色。未获得NPY抗血清与PP家族相关肽（如禽PP、牛PP、PYY、γ-MSH、FMRF-酰胺或禽PP（31-36））发生任何交叉反应的证据。NPY抗血清是N端定向的，放射免疫测定所见的NPY区域水平与免疫组织化学研究中所见的NPY免疫反应性结构的出现情况良好平行。高压液相色谱分析显示，来自大脑皮层和输精管的NPY免疫反应性物质的洗脱图谱与标准猪NPY的洗脱图谱相似。PYY抗血清主要使大鼠胰腺和肠道的内分泌细胞以及脑干中的一个小神经元系统染色。尽管该抗血清在放射免疫测定中与NPY有轻微交叉反应，但神经元PYY染色与NPY染色是分开的。在肠道中发现了高水平的PYY，在大鼠的背迷走神经复合体中也发现了高于阈值的水平。研究的其他抗血清（针对禽PP、牛PP、γ-MSH和FMRF-酰胺制备）引起的神经元染色在用NPY预吸收后被消除。即使在放射免疫测定中未发现与NPY有可检测的交叉反应，情况也是如此。这些后一种抗血清还以复杂的交叉反应关系使胰腺和肠道的内分泌细胞染色，表明除了PYY和NPY外，还存在肠道PP样肽。
