丁基- Toyopearl 650作为一种用于水溶性酶蛋白的新型疏水吸附剂。

Butyl-Toyopearl 650 as a new hydrophobic adsorbent for water-soluble enzyme proteins.

作者信息

Shin M, Sakihama N, Oshino R, Sasaki H

出版信息

Anal Biochem. 1984 Apr;138(1):259-61. doi: 10.1016/0003-2697(84)90800-5.

Abstract

Butyl-Toyopearl 650, a butyl derivative of Toyopearl HW-65, was synthesized for use in hydrophobic chromatography. Water-soluble enzyme proteins were adsorbed on butyl-Toyopearl 650 in the presence of ammonium sulfate and eluted easily in the absence of the salt. Cytochrome c, myoglobin, and chymotrypsinogen A were successfully separated on a butyl-Toyopearl 650 column in order of their individual hydrophobicity by decreasing the concentration of ammonium sulfate contained in the buffer eluant. Based on these results, the use of butyl-Toyopearl 650 is demonstrated for the hydrophobic separation of water-soluble enzyme proteins.

摘要

Butyl-Toyopearl 650是Toyopearl HW-65的丁基衍生物，合成后用于疏水色谱。水溶性酶蛋白在硫酸铵存在下吸附于Butyl-Toyopearl 650上，并在无盐条件下易于洗脱。通过降低缓冲洗脱液中硫酸铵的浓度，细胞色素c、肌红蛋白和胰凝乳蛋白酶原A在Butyl-Toyopearl 650柱上按照各自的疏水性顺序成功分离。基于这些结果，证明了Butyl-Toyopearl 650可用于水溶性酶蛋白的疏水分离。