Suzuki H, Sugimoto Y, Tanaka K, Nishimura T, Tanaka N
J Antibiot (Tokyo). 1984 May;37(5):602-9. doi: 10.7164/antibiotics.37.602.
A macromomycin (MCR)-resistant subline of mouse lymphoblastoma L5178Y cells was isolated after successive treatment of tumor-bearing mice with the antibiotic for 7 transplant generations, followed by cloning in culture in MCR-containing soft agar medium. The resistant cell line was about 17 times more resistant to MCR than was the parental cell line and exhibited cross-resistance to neocarzinostatin, mitomycin C and adriamycin in a similar degree to MCR. No significant cross-resistance was observed with aclarubicin, bleomycin and neothramycin. Alkaline phosphodiesterase activity in the plasma membrane of resistant cells was higher than that of parental cells. Uptake and efflux studies with [3H]adriamycin suggested that the resistance is due to decreased uptake and increased efflux of the antibiotic in resistant cells. Hybridization studies with MCR-sensitive and -resistant cells showed that the MCR resistance is a codominant trait in somatic cell hybrids.
在用抗生素连续处理荷瘤小鼠7代移植后，从小鼠淋巴瘤L5178Y细胞中分离出一株耐大霉素（MCR）的亚系，随后在含MCR的软琼脂培养基中进行培养克隆。该耐药细胞系对MCR的耐药性约为亲代细胞系的17倍，并对新制癌菌素、丝裂霉素C和阿霉素表现出与MCR相似程度的交叉耐药性。对阿克拉霉素、博来霉素和新霉素未观察到明显的交叉耐药性。耐药细胞的质膜碱性磷酸二酯酶活性高于亲代细胞。用[3H]阿霉素进行的摄取和外排研究表明，耐药性是由于耐药细胞中抗生素摄取减少和外排增加所致。对MCR敏感和耐药细胞的杂交研究表明，MCR耐药性是体细胞杂种中的共显性性状。
