气相色谱-质谱联用技术在人体生物蝶呤代谢研究中的应用。生物发光嗪和2'-脱氧蝶啶嗪的检测。

Application of gas chromatography-mass spectrometry to the study of biopterin metabolism in man. Detection of biolumazine and 2'-deoxysepialumazine.

作者信息

Kuster T, Matasović A, Niederwieser A

出版信息

J Chromatogr. 1984 May 4;290:303-10. doi: 10.1016/s0021-9673(01)93584-8.

Abstract

The separation characteristics of the trimethylsilyl ether derivatives of various naturally occurring and synthetic pteridines on a apolar glass capillary column, together with their mass spectra, permit their identification and quantitation in biological samples. Examples are given of the determination of the ratio of monapterin to neopterin in urine, of monitoring excreted pterin metabolites after a loading test with 6-methyltetrahydropterin in urine and of structure elucidation of lumazines , previously unknown in man. 6- Methylisoxanthopterin was shown to be the main metabolite in urine after administration of 6-methyl-5,6,7,8-tetrahydropterin. Biolumazine and 2'- deoxysepialumazine were found in human faeces after administration of ( 6R ,S)-5,6,7,8-tetrahydro-L-erythro-biopterin.

摘要

各种天然存在的和合成的蝶啶的三甲基硅醚衍生物在非极性玻璃毛细管柱上的分离特性及其质谱，使得它们能够在生物样品中被鉴定和定量。给出了测定尿中单蝶呤与新蝶呤比例的例子、监测用6-甲基四氢蝶呤进行负荷试验后尿中排泄的蝶呤代谢产物的例子，以及对人体中以前未知的lumazines进行结构解析的例子。结果表明，给予6-甲基-5,6,7,8-四氢蝶呤后，6-甲基异黄蝶呤是尿中的主要代谢产物。给予(6R,S)-5,6,7,8-四氢-L-赤藓糖型生物蝶呤后，在人粪便中发现了生物lumazine和2'-脱氧sepiolumazine。