在含有马血清的培养基中小鼠囊胚的着床期样生长与发育

Periimplantation-like growth and development of mouse blastocysts in medium containing horse serum.

作者信息

Van Winkle L J, Campione A L

出版信息

Experientia. 1984 Sep 15;40(9):988-90. doi: 10.1007/BF01946473.

DOI:10.1007/BF01946473

Abstract

Horse serum (HS) supported growth and differentiation of blastocysts as well as or better than fetal bovine serum (FBS) (p less than 0.01) as measured by a) the onset of trophoblastic outgrowth, b) the size of the resultant outgrowths, c) the size of nuclei in trophoblasts after their outgrowth and d) the size of the inner cell mass in outgrowths. Moreover, polyamines were found to be approximately 10-100 times less toxic to embryos when added to medium containing HS than when the medium contained FBS.

摘要

通过以下指标衡量，马血清（HS）对囊胚生长和分化的支持作用与胎牛血清（FBS）相当甚至更好（p小于0.01）：a）滋养层细胞开始向外生长；b）最终向外生长的大小；c）滋养层细胞向外生长后细胞核的大小；d）向外生长的内细胞团的大小。此外，当添加到含有HS的培养基中时，多胺对胚胎的毒性比添加到含有FBS的培养基中时低约10至100倍。