低钠浓度：是导致滞育小鼠囊胚对氨基酸摄取和掺入减少的一个因素？

Low Na+ concentration: a factor contributing to diminished uptake and incorporation of amino acids by diapausing mouse blastocysts?

作者信息

van Winkle L J

出版信息

J Exp Zool. 1977 Nov;202(2):275-81. doi: 10.1002/jez.1402020218.

DOI:10.1002/jez.1402020218

Abstract

Uptake and incorporation of 14C amino acids by normal and diapausing blastocysts was found to be Na+ dependent with one exception. Delayed embryos preincubated 25 h in medium containing 124 mM Na+ had labeling characteristics independent of the Na+ concentration present during labeling. Since diapausing embryos preincubated 25 h in low Na+ medium (55mM) retained their Na+ dependent labeling characteristics, delayed blastocysts may be in a low Na+ environment in situ. Low Na+ may explain why delayed blastocysts are unable to utilize radioactive methionine in vivo (Weitlauf and Greenwald, '68).

摘要

研究发现，正常胚泡和滞育胚泡对¹⁴C氨基酸的摄取和掺入除了一个例外情况外均依赖于Na⁺。在含有124 mM Na⁺的培养基中预孵育25小时的延迟胚泡具有与标记期间存在的Na⁺浓度无关的标记特征。由于在低Na⁺培养基（55 mM）中预孵育25小时的滞育胚泡保留了其依赖Na⁺的标记特征，延迟胚泡在原位可能处于低Na⁺环境中。低Na⁺可能解释了为什么延迟胚泡在体内无法利用放射性甲硫氨酸（韦特劳夫和格林瓦尔德，1968年）。