Gagelmann M, Mrwa U, Bostrom S, Rüegg J C, Hartshorne D
Pflugers Arch. 1984 May;401(1):107-9. doi: 10.1007/BF00581542.
In various skinned smooth muscle fiber preparations, (porcine carotid artery, rat tail artery, chicken gizzard and Taenia coli from guinea pig) a Ca2+-independent myosin light chain kinase (MLCK) initiated a contraction in absence of Ca2+. While the Ca2+ insensitive MLCK was effective on the vertebrate smooth muscles it did not act on the invertebrate skinned skeletal muscle preparation from Limulus and anterior byssus retractor muscle from Mytilus edulis. The results indicate that in vertebrate smooth muscles phosphorylation is sufficient for activation and that there is no obligatory role for an additional mechanism in initiation of contraction.
在各种去表皮的平滑肌纤维制剂中（猪颈动脉、大鼠尾动脉、鸡胗以及豚鼠的结肠带），一种不依赖钙离子的肌球蛋白轻链激酶（MLCK）在无钙离子的情况下引发了收缩。虽然这种对钙离子不敏感的MLCK对脊椎动物平滑肌有效，但它对来自鲎的无脊椎动物去表皮骨骼肌制剂以及紫贻贝的前足丝牵缩肌不起作用。结果表明，在脊椎动物平滑肌中，磷酸化足以激活，并且在收缩起始过程中不存在额外机制的必然作用。
