Istomin V V, Shkliarov M I
Zh Nevropatol Psikhiatr Im S S Korsakova. 1984;84(7):969-74.
In the context of the development of approaches to a quantitative automated analysis of neural tissue, the use of television image analyzers seems to be fairly promising. A complex of programmes for the television analysis system (TAS) by "Leitz Company" has been developed to implement an automated analysis of cerebral cortex sections. At the first stage of investigation, the operator sets a plan of scanning the object in the interactive regimen and with a small magnification of the microscope. The second stage involves the focusing and discrimination of images, selection of objects for analysis and measurement of the principal stereological parameters of neurons with a large magnification and at an automatic regimen. The curves obtained of changes of the stereological characteristics of the cortex in relation to the distance to the surface are in good agreement with the cytoarchitectonic pattern observed. Thus, the use of the TAS allows for the effective automated processing of neuromorphological preparations.
在神经组织定量自动分析方法的发展背景下,使用电视图像分析仪似乎颇具前景。“徕卡公司”已开发出一套用于电视分析系统(TAS)的程序组合,以实现对大脑皮质切片的自动分析。在研究的第一阶段,操作人员在交互模式下以显微镜的小放大倍数设置扫描对象的计划。第二阶段包括图像聚焦与辨别、选择分析对象以及在大放大倍数和自动模式下测量神经元的主要立体学参数。所获得的皮质立体学特征随距表面距离变化的曲线与观察到的细胞构筑模式高度吻合。因此,使用电视分析系统能够对神经形态学标本进行有效的自动处理。