Korneluk R G, Quan F, Lewis W H, Guise K S, Willard H F, Holmes M T, Gravel R A
J Biol Chem. 1984 Nov 25;259(22):13819-23.
We report the isolation and sequence of partial cDNA clones coding for human catalase. These clones were recovered from a human fibroblast cDNA library by screening with mixtures of oligonucleotide probes deduced from the amino acid sequence of human erythrocyte catalase. A comparison of their nucleotide sequence with the known protein sequence and mapping of homologous DNA sequences to the short arm of chromosome 11 in somatic cell hybrids confirmed that they coded for catalase. One of these clones contained a 462-base insertion interrupting the coding sequence with stop codons in all three reading frames. The 5' and 3' ends of the insertion correspond to the donor and acceptor consensus sequences of introns. Inspection of clones lacking the insertion confirm the location of the splice sites. We suggest this clone corresponds to the product of reverse transcription of an unspliced mRNA species. The catalase gene is the closest genetic marker mapped to Wilms tumor, one of the most prevalent of childhood cancers. Catalase cDNA probes will be useful to the examination of mitotic recombination in the etiology of this disease and may provide a useful starting point to the search for the putative Wilms tumor gene.
我们报道了编码人过氧化氢酶的部分cDNA克隆的分离及序列。这些克隆是通过用人红细胞过氧化氢酶氨基酸序列推导的寡核苷酸探针混合物筛选人成纤维细胞cDNA文库而获得的。将其核苷酸序列与已知蛋白质序列进行比较，并将同源DNA序列定位到体细胞杂种中的11号染色体短臂上，证实它们编码过氧化氢酶。其中一个克隆含有一个462个碱基的插入片段，该片段在所有三个阅读框中都用终止密码子中断了编码序列。插入片段的5′和3′末端对应于内含子的供体和受体共有序列。对缺乏插入片段的克隆进行检查证实了剪接位点的位置。我们认为这个克隆对应于未剪接mRNA物种逆转录的产物。过氧化氢酶基因是定位到肾母细胞瘤的最接近的遗传标记，肾母细胞瘤是儿童期最常见的癌症之一。过氧化氢酶cDNA探针将有助于研究这种疾病病因中的有丝分裂重组，并可能为寻找假定的肾母细胞瘤基因提供一个有用的起点。
