Steen L, Van Dessel G, De Wolf M, Lagrou A, Hilderson H J, De Keukeleire D, Pinkse F A, Fokkens R H, Dierick W S
Biochim Biophys Acta. 1984 Dec 6;796(3):294-303. doi: 10.1016/0005-2760(84)90130-9.
Inspection of the TLC pattern of the neutral lipid fraction of bovine thyroid reveals, in addition to cholesteryl esters and dolichyl fatty acid esters, the presence of a not yet identified compound in the most apolar lipid region. This unknown compound was purified on a preparative scale by silicic acid column chromatography, Lipidex-5000 gel-filtration chromatography and preparative thin-layer chromatography. By chemical (hydrolysis and reduction) and spectroscopic (ultraviolet, infrared, NMR, mass spectroscopy) methods this lipid was identified as dolichyl dolichoate. The homologue pattern was analyzed, by HPLC and FDMS, respectively.
对牛甲状腺中性脂质部分的薄层色谱图进行检查发现，除胆甾醇酯和多萜醇脂肪酸酯外，在最具非极性的脂质区域存在一种尚未鉴定的化合物。该未知化合物通过硅酸柱色谱、Lipidex - 5000凝胶过滤色谱和制备型薄层色谱进行了制备规模的纯化。通过化学方法（水解和还原）以及光谱方法（紫外、红外、核磁共振、质谱），该脂质被鉴定为多萜醇二十二酸酯。分别通过高效液相色谱和场解吸质谱对其同系物模式进行了分析。
