Pukkila P J, Yashar B M, Binninger D M
Symp Soc Exp Biol. 1984;38:177-94.
The basidiomycete Coprinus cinereus offers many advantages for molecular analyses of meiosis. Fruiting body development is highly synchronous, and chromosome behaviour can be visualized using the light microscope. Furthermore, the small genome size (3.7 X 10(7) bp/haploid nucleus) facilitates molecular cloning procedures, and genetic characterization is reasonably advanced. To analyse controls of the meiotic process, we have isolated genomic sequences which are differentially expressed during Coprinus development by comparative cDNA hybridization to cloned genomic segments. We have concluded that fruiting body morphogenesis is associated with extensive changes in steady-state RNA levels in this fungus.
灰盖鬼伞这种担子菌为减数分裂的分子分析提供了许多优势。子实体发育高度同步，并且染色体行为可以通过光学显微镜观察到。此外，其基因组较小（单倍体细胞核为3.7×10⁷碱基对）便于分子克隆操作，而且遗传特征分析也有了相当的进展。为了分析减数分裂过程的调控机制，我们通过与克隆的基因组片段进行比较cDNA杂交，分离出了在灰盖鬼伞发育过程中差异表达的基因组序列。我们得出结论，在这种真菌中，子实体形态发生与稳态RNA水平的广泛变化有关。
