Trezeguet V, Merle M, Gandar J C, Labouesse B
FEBS Lett. 1983 Jun 27;157(1):210-4. doi: 10.1016/0014-5793(83)81147-8.
The dimeric tryptophanyl-tRNA synthetase from beef pancreas has been found to activate 2 tryptophans/mol enzyme [Eur. J. Biochem. (1982) 128, 389-398]. By using quenched-flow and stopped-flow methods under presteady-state conditions, we show that only one enzyme subunit operates at a time in the aminoacylation of tRNATrp and that the transfer reaction is not the rate-limiting step in the overall aminoacylation process.
已发现来自牛胰腺的二聚体色氨酰 - tRNA合成酶每摩尔酶可激活2个色氨酸分子[《欧洲生物化学杂志》(1982年)128卷，389 - 398页]。通过在预稳态条件下使用淬灭流动法和停流法，我们表明在tRNATrp的氨酰化过程中，一次只有一个酶亚基起作用，并且转移反应不是整个氨酰化过程中的限速步骤。
