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A simple rapid biuret method for the estimation of protein in samples containing thiols.

作者信息

Pelley J W, Garner C W, Little G H

出版信息

Anal Biochem. 1978 May;86(1):341-3. doi: 10.1016/0003-2697(78)90355-x.

DOI:10.1016/0003-2697(78)90355-x
PMID:655398
Abstract
摘要

相似文献

1
A simple rapid biuret method for the estimation of protein in samples containing thiols.一种用于估算含硫醇样品中蛋白质含量的简单快速双缩脲法。
Anal Biochem. 1978 May;86(1):341-3. doi: 10.1016/0003-2697(78)90355-x.
2
Rapid and thiol-specific high-throughput assay for simultaneous relative quantification of total thiols, protein thiols, and nonprotein thiols in cells.用于同时相对定量细胞中总硫醇、蛋白质硫醇和非蛋白质硫醇的快速且硫醇特异性高通量检测方法。
Anal Chem. 2015 Jan 6;87(1):649-55. doi: 10.1021/ac503411p. Epub 2014 Dec 11.
3
Note on the estimation of protein by the biuret method with the use of a photoelectric colorimeter.关于使用光电比色计通过双缩脲法测定蛋白质的说明。
Med J Aust. 1951 Jul 28;2(4):114-5. doi: 10.5694/j.1326-5377.1951.tb68142.x.
4
ortho- and meta-substituted aromatic thiols are efficient redox buffers that increase the folding rate of a disulfide-containing protein.邻位和间位取代的芳香族硫醇是有效的氧化还原缓冲剂,可提高含二硫键蛋白质的折叠速率。
J Biotechnol. 2006 Aug 20;125(1):39-47. doi: 10.1016/j.jbiotec.2006.02.007. Epub 2006 Apr 17.
5
Quantitative measurements of the changes in protein thiols in cervical intraepithelial neoplasia and in carcinoma of the human uterine cervix provide evidence for the existence of a biochemical field effect.对人子宫颈上皮内瘤变和子宫颈癌中蛋白质硫醇变化的定量测量为生化场效应的存在提供了证据。
Cancer Res. 1990 Oct 15;50(20):6663-7.
6
Action of cadmium and thiols on tissues and enzymes.镉与硫醇对组织和酶的作用。
Arch Biochem. 1947 Feb;12(2):283-91.
7
Inhibition of the respiration of Trypanosoma rhodesiense by thiols.硫醇对罗德西亚锥虫呼吸的抑制作用。
Biochem J. 1956 Jul;63(3):475-81. doi: 10.1042/bj0630475.
8
[Method of determination of proteins with Coomassie brilliant blue G 250. I. General characteristics and comparative analysis with the biuret method and Lowry's method].[考马斯亮蓝G 250法测定蛋白质。I. 一般特性及与双缩脲法和洛瑞法的比较分析]
Boll Soc Ital Biol Sper. 1980 Jan 30;56(2):160-5.
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Thiols and the control of carbohydrate metabolism in cerebral tissues.硫醇与脑组织中碳水化合物代谢的调控
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Thiols and disulphides can aggravate peroxynitrite-dependent inactivation of alpha1-antiproteinase.硫醇和二硫化物会加剧过氧亚硝酸盐依赖的α1-抗蛋白酶失活。
FEBS Lett. 1997 Sep 15;414(3):497-500. doi: 10.1016/s0014-5793(97)01058-2.

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Kinetics of CO insertion and acetyl group transfer steps, and a model of the acetyl-CoA synthase catalytic mechanism.CO插入和乙酰基转移步骤的动力学,以及乙酰辅酶A合成酶催化机制的模型。
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Autocatalytic activation of acetyl-CoA synthase.乙酰辅酶A合酶的自催化激活
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Active acetyl-CoA synthase from Clostridium thermoaceticum obtained by cloning and heterologous expression of acsAB in Escherichia coli.通过在大肠杆菌中克隆和异源表达acsAB获得的来自嗜热栖热菌的活性乙酰辅酶A合成酶。
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