Gutkowska J, Thibault G, Cantin M, Garcia R, Genest J
Can J Physiol Pharmacol. 1983 May;61(5):449-56. doi: 10.1139/y83-069.
The effect of chronic beta-adrenergic stimulation with isoproterenol on the kallikrein content of rat submandibular gland was estimated by radioimmunoassay, by immunohistochemistry, and by analytical isoelectric focusing. Radioimmunoassays revealed that the kallikrein content of the submandibular gland was already significantly decreased after 6 days of treatment. After 21 days, the kallikrein content was 40 times lower and by 33 days an almost complete depletion was found. These findings were confirmed by immunohistochemistry. Analytical isoelectric focusing also showed that, after 33 days of treatment, the typical bands corresponding to the kallikrein isoenzymes of the submandibular gland had disappeared. These results indicate that kallikrein secretion is influenced by beta-adrenergic stimulation.
通过放射免疫测定法、免疫组织化学法和分析等电聚焦法，评估了异丙肾上腺素慢性β-肾上腺素能刺激对大鼠下颌下腺激肽释放酶含量的影响。放射免疫测定显示，治疗6天后，下颌下腺的激肽释放酶含量已显著降低。21天后，激肽释放酶含量降低了40倍，到33天时几乎完全耗尽。这些发现通过免疫组织化学得到证实。分析等电聚焦也表明，治疗33天后，下颌下腺激肽释放酶同工酶对应的典型条带消失。这些结果表明，激肽释放酶的分泌受β-肾上腺素能刺激的影响。
