Mizutani T
J Biochem. 1983 Jul;94(1):163-9. doi: 10.1093/oxfordjournals.jbchem.a134325.
Bovine liver tRNA was adsorbed on silicone-coated porous glass in 5 M NaCl, 10 mM Tris-HCl (pH 7.6) and fractionated by elution with decreasing NaCl concentrations. tRNAPro, tRNAVal, tRNAIle, tRNAThr, tRNASer, and tRNAPhe were eluted in this order. tRNA which had been digested with ribonuclease A was not adsorbed. Q beta RNA (adsorbed onto the glass in 5 M NaCl) was eluted with 1.5 M NaCl. RNA species in a crude rRNA fraction from Escherichia coli were separated into tRNA, 5S rRNA, and high molecular weight rRNA on siliconized porous glass. A half of calf thymus DNA was adsorbed on the glass in 5 M NaCl and the residual part passed through the column. The CD spectra showed that DNA and tRNA took the C-form and the A-form in 5 M NaCl, respectively. Therefore, the discrepancies of behavior of the DNA and RNA on siliconized porous glass may be related to the occurrence of these forms. The recovery of these nucleic acids from the column was 83-100%. Adsorption chromatography on siliconized porous glass may be a useful method for the separation of tRNA, rRNA, and mRNA.
牛肝tRNA在5M NaCl、10mM Tris-HCl（pH 7.6）中吸附于硅胶包被的多孔玻璃上，并用逐渐降低的NaCl浓度洗脱进行分级分离。tRNAPro、tRNAVal、tRNAIle、tRNAThr、tRNASer和tRNAPhe按此顺序洗脱。用核糖核酸酶A消化过的tRNA不被吸附。QβRNA（在5M NaCl中吸附于玻璃上）用1.5M NaCl洗脱。大肠杆菌粗rRNA组分中的RNA种类在硅化多孔玻璃上被分离为tRNA、5S rRNA和高分子量rRNA。小牛胸腺DNA的一半在5M NaCl中吸附于玻璃上，其余部分通过柱子。圆二色光谱表明，DNA和tRNA在5M NaCl中分别呈C型和A型。因此，DNA和RNA在硅化多孔玻璃上行为的差异可能与这些构象的出现有关。这些核酸从柱子上的回收率为83%-100%。硅化多孔玻璃上的吸附色谱法可能是分离tRNA、rRNA和mRNA的一种有用方法。
