Carayon P, Guibout M, Jaquet P, Lissitzky S
Ann Endocrinol (Paris). 1978;39(1):57-8.
Binding of TSH to plasma membranes purified from normal human thyroid gland is specific, saturable and dependent upon temperature, time, pH and salt concentration (standard assay conditions: 27 degrees C, 30 min, pH 7.8, 20 mM Tris). Displacement curve of 125I-TSH by unlabelled TSH shows a curvilinear relationship between B/F and B which can be interpreted by the presence of two sites with apparent binding constants about 5 nM and 500 nM and capacities of about 3 and 30 pmol/mg membrane protein, respectively. Systematic study of binding characteristics of membranes from 6 normal glands, 7 euthyroid multinodular goitres, 14 Graves' goitres and 2 toxic nodules do not show difference in the dissociation constants and capacities of sites between normal and diseased thyroid and between nodular and perinodular tissue in toxic nodules.
促甲状腺激素（TSH）与从正常人甲状腺中纯化的质膜的结合具有特异性、饱和性，且依赖于温度、时间、pH值和盐浓度（标准测定条件：27℃、30分钟、pH 7.8、20 mM Tris）。未标记的TSH对125I-TSH的置换曲线显示，B/F与B之间呈曲线关系，这可以通过存在两个表观结合常数分别约为5 nM和500 nM、结合容量分别约为3和30 pmol/mg膜蛋白的位点来解释。对来自6个正常腺体、7个甲状腺功能正常的多结节性甲状腺肿、14个格雷夫斯甲状腺肿和2个毒性结节的膜的结合特性进行的系统研究表明，正常甲状腺与患病甲状腺之间以及毒性结节中的结节组织与结节周围组织之间，位点的解离常数和容量没有差异。
