Hansson Y, Paulie S, Larsson A, Lundblad M L, Perlmann P, Näslund I
Cancer Immunol Immunother. 1983;16(1):23-9. doi: 10.1007/BF00199901.
Serum IgG fractions from a large and homogeneous group of patients with transitional cell carcinoma of the urinary bladder (TCC) were tested for their capacity to induce antibody-dependent cellular cytotoxicity (ADCC) with lymphocytes from healthy donors against a TCC-derived target cell and one derived from adenocarcinoma of the colon. Both targets have previously been shown to be of comparable susceptibility to cell-mediated lysis in vitro. Some of the IgG preparations showed strong and dose-dependent ADCC against either one or both targets, while others gave weak reactions or none at all. Similar results were obtained with IgG from a matched group of patients with prostatic carcinoma who were used as clinical controls (CC). In parallel experiments, lymphocytes taken from the two donor groups at the same time as the serum samples were tested for their direct cytotoxicity (CMC) against the two targets. CMC gave similar results to ADCC. The differences in cytotoxicity displayed by either IgG or lymphocytes from individual donors were analysed statistically, using nonparametric statistics. To avoid introducing bias due to arbitrary data selection, the entire set of results, comprising both high and low reactors, was included in the statistical assessment. ADCC of the TCC donors' IgG against the TCC target was significantly stronger than against the colon carcinoma and also significantly stronger than that of the control donors. Similarly, the TCC patients' lymphocytes displayed a significantly higher CMC against the TCC target than against the control targets. This was not seen when the lymphocytes from the patients with prostatic carcinoma were tested. When CMC and ADCC of individual donors were compared, a statistically significant correlation between these activities was seen in three of the four donor/target combinations. These results support earlier findings and suggest that a significant fraction of both the disease-related and the 'non-selective' CMC (NK) displayed by cancer patients lymphocytes against allogeneic tumor cells in vitro reflects antibody-dependent reactions.
对一大组患有膀胱移行细胞癌（TCC）且病情均一的患者的血清IgG组分进行了检测，以评估其诱导健康供体淋巴细胞针对TCC来源的靶细胞和结肠腺癌来源的靶细胞产生抗体依赖性细胞毒性（ADCC）的能力。此前已证明，这两种靶细胞在体外对细胞介导的裂解具有相当的敏感性。一些IgG制剂对其中一个或两个靶细胞均表现出强烈且剂量依赖性的ADCC，而其他制剂则反应微弱或根本无反应。以一组匹配的前列腺癌患者作为临床对照（CC），从他们身上获取的IgG也得到了类似结果。在平行实验中，与采集血清样本同时从两组供体获取的淋巴细胞针对这两种靶细胞的直接细胞毒性（CMC）进行了检测。CMC得到了与ADCC类似的结果。使用非参数统计方法对个体供体的IgG或淋巴细胞所表现出的细胞毒性差异进行了统计分析。为避免因任意选择数据而引入偏差，统计评估纳入了包括高反应者和低反应者在内的整套结果。TCC供体的IgG针对TCC靶细胞的ADCC显著强于针对结肠癌细胞的ADCC，也显著强于对照供体的ADCC。同样，TCC患者的淋巴细胞针对TCC靶细胞的CMC显著高于针对对照靶细胞的CMC。对前列腺癌患者的淋巴细胞进行检测时未发现此现象。当比较个体供体的CMC和ADCC时，在四个供体/靶细胞组合中的三个组合中观察到这些活性之间存在统计学上的显著相关性。这些结果支持了早期研究结果，并表明癌症患者淋巴细胞在体外针对异基因肿瘤细胞表现出的与疾病相关的以及“非选择性”的CMC（NK）中，有很大一部分反映了抗体依赖性反应。
