根据微粒体膜的表面性质对其进行分级分离。
Fractionation of microsomal membranes on the basis of their surface properties.
作者信息
Ohlsson R, Jergil B, Walter H
出版信息
Biochem J. 1978 Apr 15;172(1):189-92. doi: 10.1042/bj1720189.
Abstract
Partition in dextran-poly(ethylene glycol) aqueous-phase systems can be used for both membrane subfractionation and gaining information on membrane surface properties [H. Walter (1977) in Methods of Cell Separation (Catsimpoolas, N., ed.), vol. 1, pp. 307-354, Plenum, New York]. Smooth, light rough and heavy rough rat liver microsome (obtained by sucrose-density-gradient centrifugation) were subjected to countercurrent distribution in such a system. Smooth microsomal membranes had the highest, heavy rough microsomal membranes the lowest and light rough microsomal membranes an intermediate partition coefficient. The separation is based primarily on hydrophobic differences in the membrane surfaces of the three preparations and is thus due to microsomal properties not previously utilized in their fractionation. The method permits additional subfractionations of microsomes.
摘要
葡聚糖 - 聚乙二醇水相系统中的分配可用于膜的亚分级分离以及获取有关膜表面性质的信息[H. 沃尔特（1977年），载于《细胞分离方法》（N. 卡特辛波拉斯编），第1卷，第307 - 354页，普伦出版社，纽约]。将光滑的、轻度粗糙的和重度粗糙的大鼠肝微粒体（通过蔗糖密度梯度离心获得）在这样的系统中进行逆流分配。光滑微粒体膜的分配系数最高，重度粗糙微粒体膜的最低，轻度粗糙微粒体膜的分配系数居中。这种分离主要基于这三种制剂膜表面的疏水性差异，因此是由于微粒体在其分级分离中未被利用过的性质。该方法允许对微粒体进行进一步的亚分级分离。
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