West J R, Dewey S L, Pierce D R, Black A C
Ciba Found Symp. 1984;105:8-25. doi: 10.1002/9780470720868.ch2.
Three separate groups of pregnant Sprague-Dawley rats were (1) fed a liquid diet containing 35% ethanol-derived calories, or (2) pair-fed this diet containing an isocaloric amount of maltose-dextrin instead of ethanol,or (3) fed laboratory chow ad libitum. Their offspring were killed after reaching at least 60 days of age, and their brains were processed with the Timm's or horseradish peroxidase histochemical techniques. Both procedures revealed that intrauterine exposure to ethanol produced a dramatic change in the topography of hippocampal mossy fibres. An aberrant distal infrapyramidal mossy fibre terminal band was present at mid-temporal levels (where it does not normally occur). Pair-fed controls did not show the aberrant band. Rats exposed to ethanol after birth (using an artificial rearing procedure) showed even greater aberrations in the mossy fibre terminal field than rats exposed to ethanol in utero. Thus, postnatal exposure to ethanol (equivalent to the third trimester in the human) produced more striking changes in the mossy fibre terminal field than exposure to ethanol during the entire length of gestation in the rat (equivalent to the first and second trimesters in the human).
将三组怀孕的斯普拉格-道利大鼠分别进行如下处理：(1) 喂食含35%乙醇热量的流质饮食；(2) 配对喂食含等热量麦芽糖糊精而非乙醇的这种饮食；(3) 随意喂食实验室饲料。它们的后代在至少60日龄后处死，其大脑用蒂姆氏法或辣根过氧化物酶组织化学技术处理。两种方法均显示，子宫内暴露于乙醇会使海马苔藓纤维的拓扑结构发生显著变化。在颞叶中部水平出现了异常的远端锥体下苔藓纤维终末带（正常情况下该部位不会出现）。配对喂食的对照组未出现异常带。出生后暴露于乙醇的大鼠（采用人工饲养程序）在苔藓纤维终末区的异常比子宫内暴露于乙醇的大鼠更大。因此，出生后暴露于乙醇（相当于人类妊娠晚期）在苔藓纤维终末区产生的变化比大鼠整个妊娠期暴露于乙醇（相当于人类妊娠早期和中期）更为显著。
