Roshchina V V, Akulova E A
Biokhimiia. 1978 May;43(5):899-903.
Phloridzin (2',4',6',4-tetraoxyhydrochalcon-2'-glucoside) was used to study the localization of synthesis of ATP in the electrontransporting chain of photosynthesis. It was shown that phloridzin inhibits the rate of photoreduction of NADP+ by isolated pea chloroplasts by 40%, electron transport via cytochrome f by 100% and via plastocyanin--by 50%. The "crossover" experiments demonstrated that phloridzin inhibits ADP-induced photoreduction of cytochrome f, having no effect on plastocyanin under identical conditions. It is assumed that the site of ATP synthesis is localized on the reduced site of cytochrome f, while the carrier itself is located in the electron transporting chain coupled to phosphorylation. It is possible that only part of the plastocyanin molecules are located in the phosphorylating pathway of electron transport.
根皮苷（2',4',6',4-四羟基查耳酮-2'-葡萄糖苷）被用于研究光合作用电子传递链中ATP合成的定位。结果表明，根皮苷可使离体豌豆叶绿体对NADP+的光还原速率降低40%，使通过细胞色素f的电子传递速率降低100%，通过质体蓝素的电子传递速率降低50%。“交叉”实验表明，在相同条件下，根皮苷可抑制ADP诱导的细胞色素f的光还原，而对质体蓝素无影响。推测ATP合成位点定位于细胞色素f的还原位点上，而载体本身位于与磷酸化偶联的电子传递链中。可能只有部分质体蓝素分子位于电子传递的磷酸化途径中。
