[Immunogenicity of H-antigen polymers obtained by different methods].

The authors studied the properties of two polymeres prepared from flagellin by various methods: precipitation with ammonium sulfate with the subsequent centrifugation or treatment with glutaric aldehyde in solution and subsequent gel-chromatography. Molecules of the former (P-I) and of the latter (P-II) were similar by shape, but the mol wt of P-I was greater. The preparations contained common H-antigen and were similar by serological activity. P-I had a very high "priming" activity for mice. P-II was highly immunogenic only within the narrow range of doses, was less immunogenic in comparison with P-II, and, in difference from P-I, induced formation of some amount of 7S-antibodies.