Hirayama R, Sato K, Hirokawa K, Chang M P, Mishima Y, Makinodan T
Cancer Immunol Immunother. 1984;18(3):209-14. doi: 10.1007/BF00205513.
An attempt has been made to determine (a) whether aging plays an important role in resistance against metastasis and (b) whether dithiothreitol, an effective in vitro mitogenic potentiator of splenic cells of young and old mice, can modulate the occurrence of pulmonary metastasis. B16-F10 melanoma cells were injected into the outer ear of young and old female C57BL/6 mice; and the growth of the primary tumor, the palpable size of the cervical lymph node, and the number of lung metastases were then determined at various intervals. The ear was amputated when the primary tumor reached 4 mm in mean diameter. The following results were obtained. (a) The growth rate of the primary tumor in young mice is comparable to that in old mice. (b) Enlargement of the cervical lymph node occurs earlier in old than in young mice. (c) Old mice are more vulnerable to pulmonary metastases, but small metastasized pulmonary colonies are more prominent in old than in young mice. (d) Dithiothreitol (100 micrograms) injected every 2 days after the inoculation of tumor cells is effective in reducing the incidence of pulmonary metastases in old mice.
(a) 衰老在抗转移过程中是否起重要作用；(b) 二硫苏糖醇（一种对年轻和老年小鼠脾细胞均有效的体外促有丝分裂增强剂）能否调节肺转移的发生。将B16-F10黑色素瘤细胞注射到年轻和老年雌性C57BL/6小鼠的外耳中；然后在不同时间间隔测定原发性肿瘤的生长情况、可触及的颈部淋巴结大小以及肺转移灶数量。当原发性肿瘤平均直径达到4 mm时，将耳朵切除。得到以下结果：(a) 年轻小鼠原发性肿瘤的生长速度与老年小鼠相当；(b) 老年小鼠颈部淋巴结肿大比年轻小鼠出现得更早；(c) 老年小鼠更容易发生肺转移，但老年小鼠的小转移肺集落比年轻小鼠更明显；(d) 在接种肿瘤细胞后每2天注射一次二硫苏糖醇（100微克）可有效降低老年小鼠肺转移的发生率。
