四倍体人类淋巴细胞中糖皮质激素抵抗的获得减少。
Decreased acquisition of glucocorticoid resistance in tetraploid human lymphoid cells.
作者信息
Gledhill R M, Gray D A, Solberg-Scott M, Norman M R
出版信息
Mol Cell Endocrinol. 1983 Jan;29(1):67-77. doi: 10.1016/0303-7207(83)90005-9.
Resistance to dexamethasone (1 microM) was measured in glucocorticoid-sensitive diploid and tetraploid clones of the human leukaemic cell line CCRF-CEM (clone C7) during continuous culture and after X-ray or chemical mutagenesis. In continuous culture resistant diploid cells accumulated at a rate of about one cell per 10(5) divisions, while the rate for tetraploid cells was less than one per 10(7) divisions. Chemical and X-ray mutagenesis caused a marked increase in the number of resistant diploid cells but had very little effect on tetraploid cells. These results are consistent with a mutational basis for the acquisition of the glucocorticoid-resistant phenotype in human lymphoid cells.
在人白血病细胞系CCRF-CEM（克隆C7）的糖皮质激素敏感二倍体和四倍体克隆中，在连续培养期间以及经X射线或化学诱变后，测定了对地塞米松（1微摩尔）的抗性。在连续培养中，抗性二倍体细胞以约每10⁵次分裂产生1个细胞的速率积累，而四倍体细胞的速率小于每10⁷次分裂产生1个细胞。化学诱变和X射线诱变导致抗性二倍体细胞数量显著增加，但对四倍体细胞影响很小。这些结果与人类淋巴细胞中获得糖皮质激素抗性表型的突变基础一致。
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