Pontremoli S, Melloni E, Salamino F, Sparatore B, Michetti M, Horecker B L
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1261-4. doi: 10.1073/pnas.80.5.1261.
Specific inhibitors of three lysosomal proteinases are present in the cytosolic and lysosomal compartments of rabbit liver. The cytosolic inhibitors, purified by chromatography on DEAE-Trisacryl and Sephadex G-75, show specificities toward cathepsin M, cathepsins B and L, and fructose 1,6-bisphosphatase converting enzyme (CE), respectively, and are designated IM, IB/L, and ICE. Inhibitors with similar specificities have been isolated from the intralysosomal compartment. Two of these inhibitors, IM and ICE, are also present in the lysosomal membranes. The lysosomal distribution parallels that of the respective proteinases. The inhibitors are polypeptides with molecular weights of 5,000-10,000 for the two forms of IB/L, 12,500 for IM, and 10,000-40,000 for the ICE species.
兔肝脏的胞质溶胶和溶酶体区室中存在三种溶酶体蛋白酶的特异性抑制剂。通过DEAE-三乙醇胺琼脂糖凝胶和葡聚糖凝胶G-75柱层析纯化得到的胞质溶胶抑制剂，分别对组织蛋白酶M、组织蛋白酶B和L以及果糖1,6-二磷酸酶转化酶（CE）具有特异性，分别命名为IM、IB/L和ICE。已从溶酶体内区室中分离出具有相似特异性的抑制剂。其中两种抑制剂，IM和ICE，也存在于溶酶体膜中。溶酶体中的分布与各自蛋白酶的分布相似。这些抑制剂是多肽，两种形式的IB/L分子量为5000 - 10000，IM为12500，ICE种类为10000 - 40000。
