Ali H A, McLelland J
Cell Tissue Res. 1978 Jun 8;189(3):537-48. doi: 10.1007/BF00209139.
The enteric nerve plexuses of the domestic fowl (Gallus domesticus) were investigated in sections and stretch preparations by means of the cholinesterase and glyoxylic acid fluorescence histochemical techniques. Cholinesterase-positive and varicose and non-varicose fluorescent nerve fibers were distributed at all levels of the gut in myenteric, submucosal, muscle and mucosal plexuses, and in a perivascular plexus. The density of the innervation and the detailed distribution of the nerves varied in different parts of the intestinal tract. All nerve plexuses appeared to be best developed in the rectum. Whereas the circular muscle coat contained a substantial number of nerves at all levels of the gut, the longitudinal coat was well innervated only in the rectum. The major portion of the mucosal plexus appeared to be associated with the intestinal glands. The nerve cell bodies were restricted to the myenteric and submucosal plexuses and were mainly cholinesterase-positive. Fluorescent ganglion cells were not observed. Pretreatment of stretch preparations with NADH: Nitro BT to stain ganglion cells showed that the majority of the cells were surrounded by a meshwork of fluorescent varicose fibres, although none of the fibres appeared to be associated with individual cells. The perivascular plexus was mainly associated with the arteries. The functional significance of the innervation is discussed.
采用胆碱酯酶和乙醛酸荧光组织化学技术，通过切片和拉伸标本对家鸡（原鸡）的肠神经丛进行了研究。胆碱酯酶阳性、曲张和非曲张的荧光神经纤维分布于肌间神经丛、黏膜下神经丛、肌肉神经丛、黏膜神经丛以及血管周围神经丛的肠道各层。神经支配的密度和神经的详细分布在肠道不同部位有所不同。所有神经丛在直肠中似乎发育最为完善。虽然环形肌层在肠道各层都含有大量神经，但纵行肌层仅在直肠中神经支配良好。黏膜神经丛的主要部分似乎与肠腺有关。神经细胞体局限于肌间神经丛和黏膜下神经丛，且主要为胆碱酯酶阳性。未观察到荧光神经节细胞。用NADH：硝基蓝四唑对拉伸标本进行预处理以染色神经节细胞，结果显示大多数细胞被荧光曲张纤维网包围，尽管没有纤维似乎与单个细胞相关。血管周围神经丛主要与动脉相关。文中讨论了这种神经支配的功能意义。
