单克隆抗体以及与黑色素瘤细胞表面蛋白聚糖结合的抗体-毒素偶联物可抑制体内肿瘤生长。

Bumol T F, Wang Q C, Reisfeld R A, Kaplan N O

Proc Natl Acad Sci U S A. 1983 Jan;80(2):529-33. doi: 10.1073/pnas.80.2.529.

A monoclonal antibody directed against a cell surface chondroitin sulfate proteoglycan of human melanoma cells, 9.2.27, and its diphtheria toxin A chain (DTA) conjugate were investigated for their effects on in vitro protein synthesis and in vivo tumor growth of human melanoma cells. The 9.2.27 IgG and its DTA conjugate display similar serological activities against melanoma target cells but only the conjugate can induce consistent in vitro inhibition of protein synthesis and toxicity in M21 melanoma cells. However, both 9.2.27 IgG and its DTA conjugate effect significant suppression of M21 tumor growth in vivo in an immunotherapy model of a rapidly growing tumor in athymic nu/nu mice, suggesting that other host mechanisms may mediate monoclonal antibody-induced tumor suppression.

研究了一种针对人黑色素瘤细胞表面硫酸软骨素蛋白聚糖的单克隆抗体9.2.27及其与白喉毒素A链（DTA）的缀合物对人黑色素瘤细胞体外蛋白质合成和体内肿瘤生长的影响。9.2.27 IgG及其与DTA的缀合物对黑色素瘤靶细胞表现出相似的血清学活性，但只有缀合物能在M21黑色素瘤细胞中诱导一致的体外蛋白质合成抑制和毒性。然而，在无胸腺裸鼠快速生长肿瘤的免疫治疗模型中，9.2.27 IgG及其与DTA的缀合物均能显著抑制M21肿瘤的体内生长，这表明其他宿主机制可能介导单克隆抗体诱导的肿瘤抑制。