通过与蓖麻毒素A链偶联的抗体对正常或肿瘤性B细胞进行选择性杀伤。
Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.
作者信息
Krolick K A, Villemez C, Isakson P, Uhr J W, Vitetta E S
出版信息
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5419-23. doi: 10.1073/pnas.77.9.5419.
Abstract
Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface immunoglobulins on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the two allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. The results demonstrate that A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.
摘要
针对正常或肿瘤性鼠B淋巴细胞表面免疫球蛋白的高度特异性抗体（亲和纯化或杂交瘤抗体）与植物毒素蓖麻毒蛋白的A链共价偶联。此类偶联物含有针对IgM、两种IgD同种异型之一或B细胞肿瘤BCL1独特型的特异性抗体，在体外能迅速与表达相应表面抗原的细胞结合，并抑制这些细胞中的蛋白质合成。结果表明，A链偶联的抗独特型抗体可用作肿瘤特异性细胞毒剂。
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本文引用的文献
1
Increased toxicity of diphtheria toxin for human lymphoblastoid cells following covalent linkage to anti-(human lymphocyte) globulin or its F(ab')2 fragment.白喉毒素与抗（人淋巴细胞）球蛋白或其F（ab'）2片段共价连接后，对人淋巴母细胞毒性增强。
Eur J Biochem. 1980 Mar;104(2):381-90. doi: 10.1111/j.1432-1033.1980.tb04438.x.
2
3
Allelic exclusion of IgD allotypes on murine B cells.小鼠B细胞上IgD同种异型的等位基因排斥
J Immunol. 1980 Jun;124(6):2988-90.
4
Different biological properties of the two constituent peptide chains of ricin, a toxic protein inhibiting protein synthesis.蓖麻毒素是一种抑制蛋白质合成的毒性蛋白，其两条组成肽链具有不同的生物学特性。
Biochemistry. 1973 Jul 31;12(16):3121-6. doi: 10.1021/bi00740a028.
5
Affinity chromatography.亲和层析
Annu Rev Biochem. 1971;40:259-78. doi: 10.1146/annurev.bi.40.070171.001355.
6
Diphtheria.白喉
Science. 1973 Oct 26;182(4110):353-8. doi: 10.1126/science.182.4110.353.
7
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
8
Similar idiotypic specificity for the membrane IgD and IgM of human B lymphocytes.人类B淋巴细胞的膜IgD和IgM具有相似的独特型特异性。
J Immunol. 1975 Jan;114(1 Pt 1):250-2.
9
A hybrid protein containing the toxic subunit of ricin and the cell-specific subunit of human chorionic gonadotropin. II. Biologic properties.
J Biol Chem. 1979 Feb 25;254(4):1028-32.
10
Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.蛋白质巯基化与可逆蛋白质-蛋白质偶联。N-琥珀酰亚胺基3-(2-吡啶二硫基)丙酸酯，一种新型异双功能试剂。
Biochem J. 1978 Sep 1;173(3):723-37. doi: 10.1042/bj1730723.
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