Krolick K A, Villemez C, Isakson P, Uhr J W, Vitetta E S
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5419-23. doi: 10.1073/pnas.77.9.5419.
Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface immunoglobulins on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the two allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. The results demonstrate that A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.
针对正常或肿瘤性鼠B淋巴细胞表面免疫球蛋白的高度特异性抗体（亲和纯化或杂交瘤抗体）与植物毒素蓖麻毒蛋白的A链共价偶联。此类偶联物含有针对IgM、两种IgD同种异型之一或B细胞肿瘤BCL1独特型的特异性抗体，在体外能迅速与表达相应表面抗原的细胞结合，并抑制这些细胞中的蛋白质合成。结果表明，A链偶联的抗独特型抗体可用作肿瘤特异性细胞毒剂。
