Yoshikai Y, Miake S, Sano M, Nomoto K
Cell Immunol. 1983 Apr 15;77(2):266-78. doi: 10.1016/0008-8749(83)90027-8.
The effects of peritoneal exudate macrophages on antibody response to sheep erythrocytes (SRBC) were investigated in mice. Peritoneal exudate macrophages obtained from mice injected intraperitoneally with proteose peptone or Corynebacterium parvum 4 days earlier had stronger ability to phagocytize and degrade SRBC than normal resident macrophages. These macrophages suppressed antibody formation to SRBC in vitro as well as in vivo. This suppression was overridden by increasing the amount of SRBC and diminished completely by pretreatment of the macrophages with iodoacetate and partly by pretreatment with 2-deoxyglucose, both known to be inhibitors of phagocytosis, but not by addition of indomethacin to the in vitro culture. These results suggest that the suppression of antibody response by peritoneal exudate macrophages was due to the increased activity of these cells as scavenger cells, resulting in a reduced amount of effective antigenic stimulation, and that it was not mediated by a prostaglandin-dependent mechanism. The scavenger function of these macrophages may be due to Ia-negative macrophages.
在小鼠中研究了腹腔渗出巨噬细胞对绵羊红细胞（SRBC）抗体反应的影响。4天前经腹腔注射蛋白胨或短小棒状杆菌的小鼠所获得的腹腔渗出巨噬细胞，比正常驻留巨噬细胞具有更强的吞噬和降解SRBC的能力。这些巨噬细胞在体外和体内均抑制对SRBC的抗体形成。增加SRBC的量可克服这种抑制作用，用碘乙酸预处理巨噬细胞可使其完全消除，用2-脱氧葡萄糖预处理可部分消除，这两种物质均为已知的吞噬作用抑制剂，但在体外培养中添加消炎痛则不能消除。这些结果表明，腹腔渗出巨噬细胞对抗体反应的抑制是由于这些细胞作为清除细胞的活性增加，导致有效抗原刺激量减少，且不是由前列腺素依赖性机制介导的。这些巨噬细胞的清除功能可能归因于Ia阴性巨噬细胞。
